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KMID : 1377020120090040185
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Tissue Engineering and Regenerative Medicine
2012 Volume.9 No. 4 p.185 ~ p.193
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Human umbilical cord-derived mesenchymal stem cells differentiate into ligament-like cells with mechanical stimulation in various media
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Kang Mi-Na
Yoon Hee-Hoon
Seo Young-Kwon
Park Jung-Keug
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Abstract
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Applying umbilical cord mesenchymal stem cells (UC-MSCs) is extremely promising in regenerative medicine. In this study, we determined the effect of mechanical stimulation at 10% strain (5 sec of stretch and 15 sec of relaxation) and various media on differentiation of UC-MSCs into ligament-like cells over 10 days. The effects of various media, such as Dulbecco’s modified Eagle’s medium (DMEM), Advanced D-MEM (A-DMEM), and MegaCell™ DMEM (M-DMEM), were investigated. The control group was cultured in DMEM without cyclic strain. These experiments showed that the M-DMEM and A-DMEM groups were better recognized by specific interactions between extracellular matrix (ECM) molecules and membrane proteins compared to that in the DMEM and control groups. ECM production in the M-DMEM group was significantly higher than that in the other groups. Additionally, the reverse transcription polymerase chain reaction revealed that mechanical stimulation led to increased collagen-III, α-smooth muscle actin, and tenascin-C expression. Furthermore, mechanical stimulation promoted ligament-like cell differentiation of UC-MSCs, thereby inducing matrix protein expression and synthesis.
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KEYWORD
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umbilical cord, mesenchymal stem cell, mechanical stimulation, ligament-like cells, differentiation
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